Solving Sample Evaporation in PCR: A Practical Guide for Reliable Results

Sample evaporation during PCR runs remains a common yet preventable challenge in laboratories worldwide. This issue can compromise data accuracy, especially in sensitive applications like qPCR and NGS library preparation.

Key Aspects	Core Strategies	Professional Recommendations
Perfect Sealing	Use high-quality PCR plates and sealing films that match the experiment.	For qPCR applications: Choose optical quality sealing films to ensure light transmission and sealing performance. For critical applications: Use heat seal films to provide a robust sealing barrier. For versatility: Use cap strips or lids to allow space for condensation reflux.
Equipment Optimization	Ensure the thermal cover functionality of the thermal cycler is operational.	Temperature Settings: Maintain the thermal cover temperature consistently at 105°C. Regular Maintenance: Calibrate the flatness and temperature accuracy of the thermal cover.
Standardized Procedures	Follow standard operating procedures to eliminate human errors.	Proper Centrifugation: Prior to loading, centrifuge at ≥2000xg for 1-2 minutes—this is the most critical step. Avoid Disturbances: Do not open the seal casually before the reaction is complete.

Visit our product page to request free evaluation samples and see the GSBIO difference in your lab.