How to Choose 0.1mL and 0.2 mL PCR Tubes & Why GSBIO Excels in Both

What's the Core Differences?

Feature	GSBIO 0.1 mL PCR Strips	GSBIO 0.2 mL PCR Strips
Profile	Low Profile	Standard Profile
Height (Dimensional Design Value)	15.74mm	20.66mm
Well Volume	Up to 100ul	Up to 200ul
Ideal Use	Fast/qPCR	Routine PCR/qPCR
Key Advantage	Reduced dead space minimizes evaporation and boosts signal	Universal fit for the majority of thermal cycler blocks

How to Choose？

1. Start with Your Instrument: Check your thermal cycler manual first. 0.2 mL is universally compatible. If you choose 0.1 mL tubes, please check your block. Many modern cyclers offer dual blocks or adapters.

2. Define Your Primary Goal:

· Choose 0.1 mL tubes if: Your priorities are maximum speed, ultimate thermal uniformity, or conserving expensive reagents. Essential for fast qPCR, HRM, and low-volume reactions.

· Choose 0.2 mL tubes if: You need versatility, routine reliability, larger reaction volumes, or broad instrument compatibility. The default choice for >90% of standard applications.

3. Consider Your Reaction Scale: Reaction Volume Selection Guide

Tube Type	Optimal Performance Range	Acceptable Working Range	Critical Notes
0.1 mL Tubes	15 – 25 µL	10 – 30 µL	• Volumes below 10 µL increase evaporation risk significantly. • Volumes above 30 µL compromise thermal performance and risk overflow.
0.2 mL Tubes	25 – 50 µL	20 – 100 µL	• Best balance of thermal mass, reliability, and reagent economy. • Minimum 20 µL recommended to ensure consistent thermal contact.

Why Trust GSBIO？

· PCR Specialists: As a manufacturer-focused company, PCR consumables are our core expertise, not a side product.

· Vertical Integration: Controlling the entire production process allows us to guarantee lot-to-lot consistency, purity (PCR-inhibitor free), and optimal performance.

· Rigorously Validated: Every batch of GSBIO PCR tubes undergoes stringent tests for sealing integrity, thermal conductivity uniformity, and optical clarity (for qPCR).

Click here to learn more and access detailed product specifications